Sinan Güven Laboratory

Therapeutic Bioengineering

 

Sinan Güven sinan.guven@deu.edu.tr
Members
Ali Kemal Baş, MSc Student
Olcay Burçin Akbulud, MSc Student
Hamdullah Yanık, MSc Student
Melis Akal, MSc Student

 

OVERVIEW

Advances in stem cell biology and genetics with bioengineering science continuously lead breakthroughs and innovations in biotechnology. We harmonize fundamental sciences such as chemistry, biology, and physics with principles of engineering to generate innovative and effective therapeutic approaches, tissue mimicries and medical microdevices for medicine and pharmaceutical sciences. Our group focuses on bioengineering of novel native-like 3D cellular microenvironments, organ-on-a-chip platforms, and cell therapy approaches and integrates them for human health.

RESEARCH INTERESTS

Tissue and cellular microenvironment engineering: An understanding of how stem cells respond to physical and chemical stimulations from their extracellular environment is essential for utilizing the therapeutic potential of stem cells. We focus on developing novel biomaterials to construct cellular niches that can provide control over the stem cell differentiation and functionalization. We build tissues for regenerative medicine and pharmaceutical applications by designing and defining the cell-material and cell-cell interactions. Utilizing the bioengineering principles we develop bioreactor systems, tissue fabrication and assembly techniques for bottom-up and top-down tissue engineering.

Bio-mimicry organ-on-a-chip platform technologies for biomedical research: Organs-on-chips technologies provide a great platform for the investigation of fundamental mechanisms of organ physiology and disease. Such tools enable control over the physical and chemical factors of an in vitro organ models. Our group focuses on designing and engineering BioMEMS for basic research on cell physiology, in vitro disease models (e.g., neurodegenerative diseases, rare diseases, and cancer), diagnostics and pharmaceutical research.

Personalized and cellular therapies: Cell-based therapies provide unique and personalized approaches in current clinical treatments. Autologous and allogenic adult mesenchymal stem cells from bone marrow, adipose tissue, Wharton jelly and dental pulp, as well as limbal stem cells, and specialized cells (e.g., chondrocytes, hepatocytes) have been demonstrated to be effective in clinics. We develop novel therapeutic methods from bench to bedside by utilizing of cell-based therapies and intraoperative approaches in clinics.

RESEARCH HIGHLIGHTS

Biological organizations are highly hierarchical in architecture from the molecular to the macroscopic level. Such complexity in tissues is formed by building units that regulate and provide the system function. To mimic and bioengineer functional tissues, we need biomanufacturing tools that can generate and manipulate multi-scale building blocks. Bottom-up tissue engineering approaches focus on creating tissues by assembling heterogeneous building units such as biomacromolecules, cells, and cell-loaded microscale hydrogels in a multiscale manner. In our recent works, we demonstrated different cell and tissue assembly approaches utilizing acoustic, magnetic, micro robotic and bioprinting technologies to generate model platforms and tissues. Bioengineering approaches are essential for current therapeutic and diagnostic applications in medicine. Together with advances in genetics and cell biology, bioengineering tools provide key elements for novel and innovative medical technologies for human health.

 

Sinan Güven Şekil 2
Engineering platforms and systems for biofabrication of 3D tissues and microenvironments.
 Sinan Güven Şekil
Representative bioengineered tissue constructs A. Engineering of large osteogenic grafts with rapid engraftment capacity using mesenchymal and endothelial progenitors from human adipose tissue (Guven et al. 2011), B. In vitro endothelial capillaries from stromal vascular fraction (Guven et al. 2011), C. Enhanced engraftment and functionality of bio-engineered autologous dermo-epidermal skin grafts pre-vascularized with adipose-derived cells (Klar et al. 2014), D. Direct neural differentiation of mouse embryonic stem cells in 3D microenvironments, E. Biotunable acoustic node assembly of hepatic organoids (Chen et al. 2015), F. Guided and magnetic self-assembly of tunable magnetoceptive cardiac microtissues (Tasoglu et al. 2014).

Lab Members

Güven Lab. is currently seeking highly motivated PhD students. If interested, please contact sinan.guven@deu.edu.tr

Education/Research Experience
May 15 – present Assistant Professor
Dokuz Eylul University, Izmir Biomedicine and Genome Institute and Center,  Izmir, Turkey.
2014 – 2015 Postdoctoral fellow
Stanford University, School of Medicine, Canary Center for Cancer Early Detection, Stanford, CA, USA.
Stanford Neuroscience Institute, CA, USA.
2012 – 2014 Postdoctoral fellow
Harvard-MIT, health Sciences and Technology Brigham and Women’s Hospital, Harvard Medical School, MIT Media Lab, Synthetic Neurobiology, Cambridge, MA, USA.
2011 – 2012 Postdoctoral fellow
Department of Biomedicinei University Hospital Basel, Switzerland.
2011 PhD. in Biomedicine, University of Basel, Switzerland.
2006 – 2007 Visiting Scholar
Ludwig Boltzmann Institute Experimental and Clinical Traumatology, Vienna, Austria.
2006 MSc. in Biotechnology, Middle East Technical University, Ankara, Turkey.
2004 – 2007 Graduate Research Assistant
Faculty of Pharmacy, Hacettepe University, Ankara, Turkey.
2003 BS. in Chemistry, Middle East Technical University, Ankara, Turkey.
AWARDS AND RECOGNITION
• Science Academy Young Scientist Award Program (BAGEP) – 2017
• Outstanding Young Scientist Award, Turkish Academy of Sciences (TUBA-GEBIP) – 2016
• Young Scientist Award, Turkish Molecular Biology and Genetic Society – 2015
• Scientific and Technological Research Council of Turkey (TUBITAK) Reintegration Fellowship – 2015
• European Union, Leonardo da Vinci Scholarship, Ankara, Turkey – 2006
PUBLICATIONS (selected)
Full list and citations: Google Scholar: Sinan Guven
• Marsano A, Medeiros da Cunha CM, Ghanaati S, Gueven S, Centola M, Tsaryk R, Barbeck M, Stuedle C, Barbero A, Helmrich U, Schaeren S, Kirkpatrick JC, Banfi A, Martin I. Spontaneous In Vivo Chondrogenesis of Bone Marrow-Derived Mesenchymal Progenitor Cells by Blocking Vascular Endothelial Growth Factor Signaling. Stem Cells Transl Med. 2016 Jul 26. doi: 10.5966/sctm.2015-0321.
• Namkoong B, Guven S, Ramesan S, Liaudanskaya V, Abzhanov A, Demirci U. Recapitulating cranial osteogenesis with neural crest cells in 3-D microenvironments. Acta Biomaterialia, 2015 Dec 07. doi: 10.1016/j.actbio.2015.12.004
• Bouyer C*, Chen P*, Güven S*, Demirtaş T.T, Nieland T, Padilla F. Demirci U. A Bio-Acoustic Levitational (BAL) Assembly Method for Engineering of Multilayered, 3D Brain-Like Constructs, Using Human Embryonic Stem Cells Derived Neuro-Progenitors, Advanced Materials, doi: 10.1002/adma.201503916, 2015 (*equal contribution).
• Guven S, Chen P, Inci F, Tasoglu S, Erkmen B, Demirci U, Multiscale assembly for tissue engineering and regenerative medicine, Trends in Biotechnology doi: 10.1016/j. tibtech.2015.02.003
• Guven S, Lindsey J, Poudel I, Chinthala S, Nickerson MD, Gerami-Naini B, Gurkan UA, Anchan RM, Demirci U, Functional maintenance of differentiated embryoid bodies in microfluidic systems: A platform for personalized medicine, Stem Cells Translational Medicine, 2015; doi: 10.5966/sctm.2014-0119.
• Durmus NG, Tekin HC, Guven S, Sridhar K, Yildiz AA, Calibasi G, Ghiran I, Davis RW, Steinmetz LM, Demirci U, Energy-free single cell density profiling by magnetic levitation, Proceedings of National Academy of Science, 2015
• Tasoglu S, Leon CH, Gundogdu HI, Guven S, Vural T, Demirci U, Guided and magnetic self-assembly of tunable magnetoceptive gels. Nature Comm, 2014.
• Klar A, Güven S, Biedermann T, Luginbühl J, Böttcher-Haberzeth S, Meuli-Simmen C, Meuli M, Martin I, Scherberich A, Reichmann E, Enhanced engraftment and functionality of bio-engineered autologous dermo-epidermal skin grafts prevascularized with adipose-derived cells, Biomaterials, 35(19):5065-78,2014.
• Tasoglu S, Diller E, Guven S, Sitti M, and Demirci U, Untethered micro-robotic coding of three-dimensional material composition, Nature Comm,5:3124. 2014
• Helmrich U, Di Maggio N, Güven S, Groppa E, Melly L, Largo RD, Heberer M, Martin I, Scherberich A, Banfi A, Osteogenic graft vascularization and bone resorption by VEGF-expressing human mesenchymal progenitors, Biomaterials, 34(21):5025-35,2013
• Güven S, Karagianni M, Schwalbe M, Schreiner S, Farhadi J, Bula S, Bieback K, Martin I, Scherberich A, Validation of an automated procedure to isolate human adipose tissue-derived cells by using the Sepax® technology, Tissue Engineering Part C Methods, 18(8):575-82,2012.
BOOK CHAPTERS
• Chen P, Wang S, Inci F, Güven S, Tasoglu S and Demirci U, Cellencapsulating hydrogels for biosensing, Editors: Khademhoseini A, Demirci U, Gels Handbook: Fundamentals, Properties, Applications, World Scientific Publishing, 2015.
• Tasoglu S*, Gurkan UA*, Guven S, Demirci U, Organ Printing and Cell Encapsulation, Editors: Migliaresi C, and Motta A, Scaffolds for Tissue Engineering: Biological Design, Materials and Fabrication, Pan Stanford Publishing, 2014.
• Asghar W, Shafiee H, Chen P, Tasoglu S, Guven S, Gurkan UA, Demirci U, In Vitro Three-Dimensional Cancer Culture Models, Editors: Bae YH, Mrsny MJ, and Park K Cancer Targeted Drug Delivery, Springer, 2013 *equal contribution
PATENTS
• Scaffold-free 3D cell assembly based on patterned hydrodynamic drag force. Patent application filed, Stanford School of Medicine, 2014.
• Microfluidic system for functional maintenance of cells for personalized medicine. Patent application filed, Brigham and Women’s Hospital, 2014.